PREPARATION, CHARACTERIZATION AND IN-VITRO CELL VIABILITY ASSAY OF CHRYSIN LOADED SOLID LIPID NANOPARTICLES AS DRUG DELIVERY SYSTEM

Abstract

The aim of the present study is to prepare and characterize Solid Lipid Nanoparticles (SLNs) encapsulated Chrysin (CN) to improve the bioavailability of the drug. Chrysin-loaded solid lipid nanoparticles (CN-SLNs) with an average particle size of 240.0± 4.79nmand a total drug content of 71.10 ± 3.12% was produced using a hot homogenization followed by micro emulsion technique. The prepared CN-SLNs were also characterized using zeta potential, entrapment efficiency, X-Ray Diffraction (XRD), Transmission Electron Microscope (TEM) and cytotoxicity assay. The particles were found to be spherical in shape, with high drug entrapment of 86.29 ± 3.42%. Zeta potential studies confirmed the stability of the CN-SLNs preparation as indicated by the negative charge (-40.4 ± 2.54 mV). Whereas the XRD studies showed reduced crystallinity and the particles showed less cytotoxic effect when compared with other polymeric nanoparticles (IC₅₀ value = 25.0 ± 2.16 µg/ml).The in-vitro release kinetics showed a maximum drug release of 88.80 ± 3.05% for CN-SLNs and 39.28 ± 3.19% of Chrysin suspension(CN–S) in 72 hrs, which ensures the controlled drug release property of CN-SLNs.