ANTIDIABETIC ACTIVITY OF DIFFERENT FRACTIONS OF HYDROALCOHOLIC EXTRACT OF MNESITHEA GRANULARIS IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Abstract

Diabetes mellitus is a chronic metabolic disorder affecting a major proportion of the population worldwide. The present study was aimed to evaluate the antidiabetic effect of n-Hexane, Ethyl acetate, Ethyl acetate: Methanol (50:50) and Methanolic fractions from hydroalcoholic extract of Mnesithea granularis in streptozotocin induced diabetic rats. Among the fractions tested, methanolic fraction shown more significant antidiabetic activity and antidiabetic activity was in the order of MF>EMF>EAF>NHF. So MF was investigated further for its action on insulin, Hb, HbA1c, oxidative parameters, body weight and cell integrity of pancreas. Results indicated that animals treated with MF shown significant decrease in blood glucose, HbA1c, malondialdehyde levels and significant increase in insulin, Hb, SOD, catalase, reduced glutathione and body weight. It could be concluded that methanolic fraction of hydroalcoholic extract of Mnesithea granularis has favourable effect in bringing down the severity of diabetes however necessary studies are required on characterization of active principles.