COMPARATIVE EVALUATION OF PHYTOCOMPONENTS PRESENT IN THE METHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ., TERMINALIA BELLIRICA ROXB., AND PHYLLANTHUS EMBLICA L., FRUIT EXTRACTS USING GC-MS ANALYSIS

Abstract

Terminalia chebula, Terminalia bellirica and Phyllanthus emblica are known as Haritaki, Bibhitaki and Amlaki and involve in the holistic system of medicine, used by natural healers. The main aim of the study was the identification of phytochemicals present in the methanol fruit extracts and to evaluate their major compounds present and its nature and activities with the aid of GC-MS technique which may provide an insight to the researchers. Powdered fruit sample was extracted with Methanol as solvent using Soxhlet apparatus. GC-MS analysis of fruits extract were performed using Perkin-Elmer GC Clarus 500 system and gas chromatograph interfaced to Mass Spectrometer equipped with a Elite-5MS (30mx250µm) composed of 5% Phenyl 95% dimethylpolysiloxane. Chromatogram showing total of 24, 26 & 27compounds was identified from mesocarp and pericarp fruits. Phytochemicals had the universal properties like 1,2,3 Benzenetriol, 2-Furancarboxaldehyde, D-Allose, Furfural, n-Hexadecanoic acid, 13-Octadecenal, (Z)-, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, Levoglucosenone, Phenol, Octadecanoic acid, 2,4-Dihydroxy-2,5-dimethyl-3(2H)-furan-3-one, 1,3,5-Triazine-2,4,6-triamine, 1,6,3,4-Dianhydro-2-deoxy-a-d-lyxo-hexopyranose, 1H-1,3-Diazepine, 4,5,6,7-tetrahydro-2-methyl, Butanedioic acid, hydroxy-(S)-, 5-Oxotetrahydrofuran-2-carboxilicacid, methyl ester, 2,4(1H,3H)-Pyrimidinedione, 6-(hydroxymethyl)-1,3-dimethyl and 1,6-Anhydro-a-D-glucofuranose. This study possesses several bioactive compounds have been evaluated and justifies their uses for various ailments by traditional practitioners and logical approach for drug formulation, discovery and it may open a door to the society.