PARTIAL PURIFICATION OF PHYTASE FROM Hypocrea lixii SURT01, A POULTRY ISOLATE

Authors

  • R. THYAGARAJAN Department of Biotechnology, Faculty of Bio and Chemical Engineering, Sathyabama University, Chennai, Tamilnadu, India.
  • S. KARTHICK RAJA NAMASIVAYAM Department of Biotechnology, Faculty of Bio and Chemical Engineering, Sathyabama University, Chennai, Tamilnadu, India.
  • G. NARENDRAKUMAR Department of Biotechnology, Faculty of Bio and Chemical Engineering, Sathyabama University, Chennai, Tamilnadu, India.

Keywords:

Phytase, Purification, Ammonium sulphate, Gel permeation chromatography, SDS PAGE.

Abstract

In the present study, Hypocrea lixii SURT01, a isolate from poultry soil was subjected to 18s rRNA analysis was submitted to NCBI – Genbank. The strain was induced in minimal media which was found to produce the phytase enzyme. The characterization of extracellular phytase showed optimum activity at pH of 6.5 and temperature of 50ºC after an incubation for 120 hours. Different nitrogen sources were screened and peptone showed more enhancements in the enzyme production. Time course study was done on the culture media and that showed 120 hours to reach maximum activity. The enzyme was partially purified by 60% by ammonium sulphate precipitation and gel permeation chromatography was done to get different fractions. SDS PAGE was carried out to understand the molecular weight of the protein.

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Published

31.12.2014

How to Cite

R. THYAGARAJAN, S. KARTHICK RAJA NAMASIVAYAM, & G. NARENDRAKUMAR. (2014). PARTIAL PURIFICATION OF PHYTASE FROM Hypocrea lixii SURT01, A POULTRY ISOLATE. International Journal of Pharma and Bio Sciences, 5(4), 680–687. Retrieved from https://ijpbs.net/index.php/journal/article/view/3800

Issue

Volume 5, Issue 4, 2014

Section

Research Articles

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