INVESTIGATION ON THE NEUROPROTECTIVE EFFECTS OF HESPERIDIN ON BEHAVIOURAL ACTIVITIES IN 6-OHDA INDUCED PARKINSON MODEL

Abstract

The present study was carried out to evaluate the neuroprotective activity of hesperidin on Parkinson induced experimental animals. Parkinson disease (PD) is one of the neurodegenerative disease and oxidative stress plays a vital role in its causation. Group 1: Animals were treated normally and served as controls. Group 2:  Rats were infused with 6-hydroxydopamine (8μg/2μl in 0.1% ascorbic acid-saline) in right striatum once and were maintained for the development of Parkinson’s disease for 45 days. Group 3: Rats were infused with 6-hydroxydopamine (8μg/2μl in 0.1% ascorbic acid-saline) in right striatum once and were maintained for development of Parkinson’s disease for 3weeks. From 22nd day Hesperidin (50 mg/kg b.w) dissolved in distilled water was given for next 24 days. Group 4: Rats were infused with 6-hydroxydopamine (8μg/2μl in 0.1% ascorbic acid-saline) in right striatum once and were maintained for the development of Parkinson’s disease for 3weeks. On the 22nd day Hesperidin (50mg/kg b.w) and L-Dopa (100mg/kg b.w) dissolved in distilled water was given for the  next 24 days. Group5:Rats were infused with 6-hydroxydopamine (8μg/2μl in 0.1% ascorbic acid-saline) in right striatum for once and the rats were maintained for  the development of Parkinson’s disease for 3 weeks. On the 22nd day (L-DOPA 100mg/kg b.w) were given for next 24days. Neurological activities such as Grip test, rotational test, Swing and climb test, catalepsy etc., were studied. After 45 days body weight was noted and the animals were killed  and  blood glucose, protein and triglycerides in serum of rat animals were estimated.  The deficits in behavioral activity due to 6-OHDA lesioning were significantly restored by hesperidin  and L-Dopa combination. This study indicates that a combination of hesperidin and L-Dopa might be helpful in attenuating 6-OHDA induced lesioning in rats.