ANTIOXIDANT, ANTIPROLIFERATIVE ACTIVITIES AND GC-MS ANALYSIS OF METHANOL EXTRACT OF LEAVES OF HERACLEUM SPRENGELIANUM LINN.

Abstract

The current study was aimed at evaluation of Antioxidant & Anti proliferative potential of the methanol extract of Heracleum sprengelianum leaves and to elucidate its phytochemical profile using GC-MS analysis. The methanol extract of   leaves of H. sprengelianum as screened for its antioxidant potential by dot-plot assay, DPPH radical scavenging assay and hydroxyl radical scavenging assay. Since the results were quite encouraging, it stimulated the investigators to proceed further to screen the phytochemical profiles by qualitative and quantitative methods which were further extended to thin layer chromatography, GC-MS analysis and antiproliferative activity. The result indicates that the methanol extract of leaves of H. sprengelianum showed significant radical scavenging activity, potent toxicity to liver cancer cells and also less toxicity to normal cells. The GC-MS result showed the presence of important active metabolites such as fatty acids and phenolic acids. Since the results are promising, H. sprengelianum could be considered as a significant source of Antioxidant and Anti proliferative agents.