GC-MS PROFILING OF ETHANOLIC EXTRACT OF MORINGA OLEIFERA LEAF

Abstract

To analyze and characterize the phytochemical compounds of ethanolic leaf extract oforinga oleifera  using GC-MS. The phytochemical screening of ethanolic extract was carried out according to standard procedures stated by WHO guidelines. The various bioactive compounds of the extract weredentified by GC-MS technique.The results of the GCMS analysis revealed 35 compounds in the ethanolic extract of oringa oleifera leaves. Among the 35 compounds the following 15 major compounds revealed   iththe maximum peak percentage area in parenthesis shown in table 1 :  1,2,3-Cyclopentanetriol  1.63), L-Galactose, 6- eoxy (4.35), n-Hexadecanoic acid (28.84), Tetradecanoic acid (2.12), cis- accenic acid (26.45), Octadecanoic acid (4.91), Palmitoyl chloride (1.56), 3-Chloro-N- sochroman-1-ylmethyl-propionamide (5.61), 2-Butenoic acid, 2-methoxy-3-methyl-, methyl ester  1.77) , 3,4- ichlorobenzonitrile (2.21), Mannitol,1,4-di-O-methyl-, tetraacetate (1.09), beta.-l- amnofuranoside, 5-O-acetyl-thio-octyl (1.01), Vitamin E(2.07), gamma.-Sitosterol (2.23), Pregn- ,7-diene-3-ol-20-one (1.47). Their respective retention times being as. follows: 13.03,15.28,18.62,18.67,20.35,20.52,21.62,23.36,23.46,23.67,23.90,23.94,27.85,29.16 29.26. The minor compounds are 4,5-Diamino-2- ydroxypyrimidine (0.45), 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (0.93), enzofuran,2,3-dihydro (0.64), Benzeneacetonitrile,4-hydroxy (0.64), 2- Cyclohexenone,4-cetamido(0.68),4-((1E)-3-Hydroxy-1-propenyl)- - ethoxyphenol(0.39), Cyclopropaneoctanal,2- ctyl (0.23), 5-Eicosene, (E) (0.54), Octadec-9-enoic acid (0.74), 5-Eicosene, (E) (0.54), Oxirane, tetradecyl (0.64), trans-13-Octadecenoic acid (0.41), 6- ctadecenoic acid, (Z)  (0.28), cis-9- exadecenal (0.21), Cyclopentadecanone,2-hydroxy (0.25), Cyclopropaneoctanal,2-octyl (0.26),  leic Acid (0.35), Oleylalcohol, heptafluorobutyrate (0.90),7- entadecyne (0.70),trans-13- ctadecenoic acid (0.60).The ethanolic leaf extract of Moringa oleifera proved to be a reservoir of bioactive compounds identified by GC-MS which could prove effective in the treatment of various diseases.