ANTIMICROBIAL EFFECT OF ANNONA SQUAMOSA ETHYL ACETATE EXTRACT

Abstract

Annona squamosa has been known to possess wide range of biological activites. The present study was focused on investigating the antibacterial property of the ethyl acetate extract from the seeds of Annona squamosa using broth dilution assay against several human pathogenic gram negative and gram positive bacterial strains. Minimum inhibitory concentration of Annona squamosa ethyl acetate extract (ASEA) on gram negative bacteria Escherichia coli, Salmonella typhi, Proteus mirabilis, Klebseilia pnemonia were found to be 19.5 µg/ml, 20.6 µg/ml, 35 µg/ml, 61.5±0.9 and gram positive bacteria Staphylococcus aureus, Bacillus subtilis were found to be 67.8µg/ml, 75 µg/ml, 27.6 µg/ml respectively. Results showed that ethyl acetate extract showed antibacterial activity to all the gram negative and gram positive bacteria. This study suggests that ethyl acetate extract of Annona squamosa was used as an antimicrobial agent.