BIOSYNTHESIS AND CHARACTERIZATION OF COPPER NANOPARTICLES FROM ENTEROCOCCUS FAECALIS

Abstract

This investigation discuss about biogenic synthesis of copper nanoparticles from standardized Enterococcus faecalis culture supernatant with 100mM copper sulfate (v/v ratio) solution. The organisms show a unique potential in environmentally friendly production of nanoparticles with different sizes. The extracellular presence of copper nanoparticles was characterized by means of UV-Visible spectrum, Field Emission Scanning Electron Microscopy (FeSEM), Energy-dispersive X-ray (EDX) and Nanoparticle Tracking Analysis (NTA). FeSEM analysis studies confirmed the formation of well-dispersed copper nanoparticles with average particle size was formed to be in the range of 20 to 90nm. NTA analysis studies also confirmed the concentration of the particles in the range of 6.52×10¹⁰ particles/ml. We also studied the antibacterial effect of biogenic copper nanoparticles against multidrug resistant pathogens like E. coli, Klebsiella pneumoniae, Methicillin Resistant Staphylococcus aureus (MRSA) and with standard cultures by modification of the agar well diffusion method.