FORMULATION AND EVALUATION OF MANGO GUM MICROSPHERES AS A TARGETED DRUG DELIVERY TO COLON.

Abstract

Present work was aimed for the design of phosphated cross-linkedmicrospheres ofmongo gum (MG) by emulsification method using sodium-tri-meta phosphate (STMP) as a cross-linking agent for treatment of colon cancer using Methotrexate (MTX) as model drug.At a stirring speed of 1000rpm and about 5h of stirring time was found to be optimal to obtain reproducible microspheres.The particle size was increased as there was increase in polymer concentration and decrease in particle size take place as there is increase in stirring speed. Emulsification method was employed for the preparation of  Cross-linked MG microspheres. At an surfactant concentration of 2% reproducible microspheres were obtained.  SEM studies showed that the drug-loaded microspheres were non-aggregated and in spherical shape. DSC and FTIR studies showed that drug and excipients are compatible. Release studies showed that drug release was more profound in cecal medium induced with enzymes causing degradation of the MG than that of the release showed in SIF. Stability studies showed that there were no significant changes in the drug content and physical appearance of microspheres.