DIRECT REGENERATION FROM EMBRYO CULTURES OF LYCOPERSICON ESCULENTUM MILL CV PUSA RUBY

Abstract

Embryos of Lycopersicon esculentum Mill cv Pusa Ruby cultured on MS medium
supplemented with auxins such as IAA (1.0mg/l) and NAA (1.0mg/l) alone produced
whole plantlets. However, embryos cultured on 2,4-D (1.0mg/l) produced callus with
roots. BAP (1.0mg/l) and Kn (1.0mg/l) alone are able to induce shoots with callus.
Among various combinations of auxins and cytokinins i.e., (1.0 mg/l) IAA + BAP (2.0
mg/l) and IAA (1.0 mg/l) + Kn (2.0 mg/l) gave good response and produced green
nodular callus with shoots. Whereas, NAA (1.0mg/l) + BAP (2.0mg/l) and Kn (2.0mg/l)
produced callus only. The combinations of IAA (0.5mg/L) + BAP (4.0 mg/L) was found
to be better medium compared to all other concentrations of IAA + Kn medium
analyzed.