Novel Method For The Detection Of HIV Using RP 24 Recombinant Protein From E. Coli BL-21

Authors

  • S. C. SINGH Inverness Medical Shimla, Shoghi Industrial Estate, Shoghi, Shimla, India
  • M. VASUDEVAN Inverness Medical Shimla, Shoghi Industrial Estate, Shoghi, Shimla, India
  • RICHA Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India
  • R. P. SINHA Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India

Keywords:

ELISA, rp24, synthetic peptides, ultrafiltration

Abstract

A novel method for the purification of recombinant protein 24 (rp24) of human immunodeficiency virus type 1 (HIV- 1) from Escherichia coli is described. The protein was over-expressed in E. coli (BL21 lDE3) cells to the levels of ~ 30 % total cell protein in the soluble fraction. The recombinant protein was extracted and purified to near homogeneity by ion-exchange steps followed by ultrafiltration. The recombinant protein in combination with synthetic peptides from immuno-dominant stretches derived from the ‘envelope’ proteins of HIV-1 and HIV-2 was used for the development of an ELISA kit, for detection of antibodies against the virus in human serum and/or plasma. 

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Published

31.12.2010

How to Cite

S. C. SINGH, M. VASUDEVAN, RICHA, & R. P. SINHA. (2010). Novel Method For The Detection Of HIV Using RP 24 Recombinant Protein From E. Coli BL-21. International Journal of Pharma and Bio Sciences, 1(4), 287–294. Retrieved from https://ijpbs.net/index.php/journal/article/view/362

Issue

Volume 1, Issue 4, 2010

Section

Research Articles