STUDY ON PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF BACTERIAL SPECIES ISOLATED FROM DECAYED FRUITS

Abstract

Most of the methods adopted for processing of decayed fruits and degradation of
wastes are not economical. In order to achieve 100% resource recovery from wastes, a
novel type of waste treatment is to be followed, so that, the recovered byproducts could
be used as biofertilizers. The objective of this study is identification of bacteria for
studying their phenotypic and genotypic characters using molecular tools and
conversion of wastes into biosolids as useful byproducts. Decayed fruit samples were
procured and pure culture of bacterial strains were isolated. The isolated strains were
subjected for phenotypic analysis of biochemical tests and genotypic gene expressions
of 16S rRNA amplification, after which, the bacterial species identified were two strains
of Bacillus subtilis, Serratia marcescens, Arthrobacter nicotianae and Corynebacterium
singulare. The antibiogram activity was performed in all the cultures. From this
analysis, strains were found to be resistant to most of the antibiotics.