DETECTION OF CIRCULATING AUTOANTIBODIES TO HA-RECEPTORS IN HUMAN LUNG CANCER

Authors

  • VENKATRAO. NUNNA Department of Studies in Biochemistry, University of Mysore, Manasagangothri, Mysore.
  • BANERJEE. SD Tufts University-School of Medicine, Boston, USA
  • KARUNA KUMAR. M Department of Studies in Biochemistry, University of Mysore, Manasagangothri, Mysore.

Keywords:

circulating autoantibodies. HA-receptors, hyaluronic acid, lung cancer, biomarker

Abstract

Identification of circulating autoantibodies to hyaluronan-receptors in lung cancer holds
great promise and these HA-receptors might be a potential biomarker, but remains
technically challenging. Interaction and involvement of hyaluronan with specific cell
surface receptors such as CD44, RHAMM and with intracellular HABP in modulating
cellular behavior has been predicted. In the present study, we detected autoantibodies
and their corresponding antigens (HA-receptors) by immunoblotting and sensitive
ELISA. Identified antigens (HA-receptors by immunoaffinity purified autoantibody IgM)
whether have binding motifs to HA or not, were further confirmed by competition
experiments. HA abolish 91% binding of autoantibodies to it’s receptors. These results
implicate that autoantibody IgM reacting proteins might be a HABPs. In conclusion,
identification of circulating tumor antigens (HA-receptors) or their related autoantibodies
provide a means for early detection and diagnosis of cancer.

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Published

31.03.2014

How to Cite

VENKATRAO. NUNNA, BANERJEE. SD, & KARUNA KUMAR. M. (2014). DETECTION OF CIRCULATING AUTOANTIBODIES TO HA-RECEPTORS IN HUMAN LUNG CANCER. International Journal of Pharma and Bio Sciences, 5(1), 1024–1032. Retrieved from https://ijpbs.net/index.php/journal/article/view/3222

Issue

Volume 5, Issue 1, 2014

Section

Research Articles

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