IN-VITRO BIOACTIVITY OF MARINE SEAWEED, CLADOPHORA RUPESTRIS

Abstract

Marine algae are rich source of biologically active compounds. This study was done to
check the antioxidant, antimicrobial and antidiabetic activity of Cladophora rupestris. In
phytochemical analysis, lipid, protein, alkaloid, flavonoids are found to be present. In the
antioxidant activity, methanol, ethyl acetate & ethanol extract showed inhibition of 78%,
72% & 63% in 5 mg/ml respectively. Well diffusion method was done to check the
antimicrobial property against human and shrimp pathogenic bacteria. MIC test was done
to check the inhibition of the pathogenic bacteria at minimum concentration of crude
extracts. In α-amylase inhibitory assay, methanol, ethanol and ethyl acetate extract
showed highest inhibition of 72%, 65% & 70% at 1000 μg/ml. In α-glucosidase inhibitory
assay, methanol and ethyl acetate showed highest inhibition of 67%, 61% and 64% at
1000 μg/ml.