IN VITRO EARLY ONTOGENIC STUDIES OF ACTINIOPTERIS RADIATA (SWARTZ) LINK. UNDER DIFFERENT MEDIUM
Keywords:
Actiniopteris radiata, in vitro spore germination, ontogenic stages of development.Abstract
Actiniopteris radiata (Swartz) Link. is a fern belonging to the family Pteridaceae. The spores collected from the pinnae of A.radiata was inoculated in Knudson’s C and Knop’s medium at pH 5.8. The ontogenic pattern such as germination type, prothallial development and micromorphological changes were studied in detail. The germination of the spore was Anemia type. The gametophyte development was Ceratopteris type. The growth area in Knudson’s C medium(KC), Knop’s (Kn) medium and brick pieces supplemented with Knudson’s C medium showed different germination percentage, growth area and micromorphology. In micromorphology, the apical notch formation varied in different medium. Few antheridiate prothalli could be seen in Knop’s medium.
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