TOPOGRAPHICAL IMAGING OF ANTIGEN – ANTIBODY INTERACTION AND HEPTATIS B SURFACE ANTIGEN ON SELF ASSEMBLED MONOLAYER OF MICA

Authors

  • NAZEEMA BANU BASHEER Department of Biotechnology, Periyar Maniammai University, Vallam, Thanjavur -613 403, India.
  • RAJASREE SHANMUGANATHAN CoRx Lifesciences and Pharmaceutical (CLAP) Pvt Ltd, Tiruchirapalli -620 020, India.
  • GNANAMBIGAI PALANICHAMY CoRx Lifesciences and Pharmaceutical (CLAP) Pvt Ltd, Tiruchirapalli -620 020, India.
  • TAMILMANI EEVERA Department of Plant Breeding & Genetics, Anbil Tharmalingam Agricultural College & Research Institute, Tami Nadu Agricultural University, Trichirapalli– 614 009, India.
  • KUMARAN SHANMUGAM Department of Biotechnology, Periyar Maniammai University, Vallam, Thanjavur -613 403, India.

Keywords:

Atomic Force Microscopy, Antigen, Antibody, Heptatis B surface antigen

Abstract

Patterning of antigen and antibody on a suitable substrate is essential for developing immunobiosensor and to study the immunocomplex formed by interaction of antigen and antibody on nitrocellulose membrane using Atomic force microscopy (AFM). The antigens were immobilized over nitrocellulose membrane and the immunoglobulins were allowed to react over the membrane containing the antigens. Analysis of the height histograms and the topography revealed the parameters such as size (24.61 to 63.61 nm) and molecular interaction between the antigens and antibodies. Heptatis surface antigen B (HBsAg) at an optimized concentration of 5 ng/ml (1:200 dilution) was successfully positioned on self assembled monolayer and its topography and surface were imaged. 

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Published

31.03.2014

How to Cite

NAZEEMA BANU BASHEER, RAJASREE SHANMUGANATHAN, GNANAMBIGAI PALANICHAMY, TAMILMANI EEVERA, & KUMARAN SHANMUGAM. (2014). TOPOGRAPHICAL IMAGING OF ANTIGEN – ANTIBODY INTERACTION AND HEPTATIS B SURFACE ANTIGEN ON SELF ASSEMBLED MONOLAYER OF MICA. International Journal of Pharma and Bio Sciences, 5(1), 319–325. Retrieved from https://ijpbs.net/index.php/journal/article/view/3133

Issue

Volume 5, Issue 1, 2014

Section

Research Articles

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