ANTIMICROBIAL ACTIVITY OF MARINE ACTINOMYCETE STREPTOMYCES DHINAKARAN 2011 (JF751041)

Abstract

Fourteen actinomycetes were isolated from soil collected from five different coastal sites of Tamil Nadu. The actinomycete designated as “A” was selected as the most potent actinomycete. Among different solvents used, ethyl acetate was found to be more effective in extraction of bioactive compounds. Minimum Inhibitory Concentration of extract was found to be 35.6µg/ml for Klebsiella pneumoniae. The actinomycete “A” strain had the ability to produce enzymes and the stability of extract was ideal at 8⁰C for 30 days. The stability of the extract was drastically reduced above 60⁰C and below pH 6. 16S rRNA sequence of the strain A showed 99% similarity with Streptomyces sp. 2438. The accession number (JF751041) for the sequence of strain A was obtained from NCBI. Based on the phylogenetic evaluation, the actinomycete was designated as Streptomyces dhinakaran 2011, which was capable of producing extracellular bioactive metabolites, that inhibits the growth of Klebsiella pneumoniae and other bacterial and fungal pathogens.