ANTIBIOFILM FORMATION AND WOUND HEALING ACTIVITY OF GLYCOPEPTIDE ANTIBIOTIC VANCOMYCIN AGAINST SKIN PATHOGENS

Abstract

Biofilm formation of Staphylococcus aureus and Staphylococcus epidermidis were determined by the production of protease and lipase. Inhibition of lipolytic and proteolytic activity will lead to control of virulence factors produced by S. aureus and S. epidermidis. Antibiofilm activity of isolated compound Vancomycin against S. aureus and S. epidermidis showed significant results. The effect of Vancomycin on Hydrophobicity Index (HI), dispersion on biofilm formation by microscopic method and glycocalyx production shows complete eradication of the biofilm formation. Vancomycin used in this study showed biofilm dispersion and disruption of the biofilm of the architecture by reducing Exopolysaccharide (EPS) production and HI. The compound were studied for its wound healing property in rats and showed significant results compared to standard drug. The inhibitor used would have great clinical significance given to the difficulties encountered with treating S. aureus and S. epidermidis biofilm centered human infections