METHOD DEVELOPMENT AND VALIDATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN PHARMACEUTICAL DOSAGE FORM

Abstract

A simple, rapid and precise method was developed for the simultaneous determination of Valsartan (VAL) and Hydrochlorothiazide (HTZ) in tablet dosage form. Analysis was performed on a C18 (125 x 4 mm, 5 μm) column with acetonitrile: phosphate buffer (pH 3) 45:55 (v/v) as mobile phase, a flow rate 1.0 mL/min, column temperature 30ºC and UV detection at 250 nm. Both the drugs were well resolved on the stationary phase and the retention time for hydrochlorothiazide was 3.124 min and for valsartan 6.469 min. The calibration curves were linear in the concentration range of 5.00-50.00 μg/mL for VAL and 1.0-10.0 μg/mL for HTC. Intra- and inter-day relative standard deviations for both the components were <2.0%. The percentage recoveries obtained for VAL and HCT ranges from 99.28 % to 100.3 %.