HPLC ANALYSIS AND PHARMACOKINETIC STUDY OF MANGOSTIN AFTER ORALLY ADMINISTRATION IN RATS

Abstract

A simple sensitive isocratic method for the detection and quantification of mangostin in plasma has been developed.  The assay consisted of reversed-phase HPLC with ultraviolet detection.  Separation was achieved on a C₁₈ reversed-phase column.  The mobile phase consisting of methanol and water (95:5 %v/v) was delivered at a flow rate of 1.0 ml/min.  The assay was shown to be linier over the range 4-100 µg/mL (r ≥ 0.9998).  The HPLC analysis has been successfully applied to pharmacokinetic studies of mangostin after oral administration at a dose 40 mg/kgbwt to rats.  The main pharmacokinetic parameters were: t_½ 7.24 h ; K el 0.058/h ; t _max 62.99 min ; C_max 4.79 µg/mL; AUC 702.45 µg min/mL, respectively.