HIGH FREQUENCY SHOOTS REGENERATION FROM IN VITRO RAISED SEEDLINGS OF MURRAYA KOENIGII (L.) SPRENG.

Authors

  • UZMA RANI Lab No-5, Biotech lab, Department of Botany, University of Rajasthan, Jaipur , India
  • NAJMA ISMAIL Lab No-5, Biotech lab, Department of Botany, University of Rajasthan, Jaipur , India
  • GARIMA ZIBBU Lab No-5, Biotech lab, Department of Botany, University of Rajasthan, Jaipur , India
  • AMLA BATRA Lab No-5, Biotech lab, Department of Botany, University of Rajasthan, Jaipur , India

Keywords:

Murraya koenigii, multiple shoots, nodal explant.

Abstract

Immature seeds of Murraya koenigii (L.) were allowed to grow in in vitro condition on ½ strength
Murashige and Skoog (MS) medium. Cotyledonary node of 3-4 weeks old seedlings were taken and
further cultured on Murashige and Skoog (MS) medium supplemented with BAP (5.0 mg/l) for multiple
shoots with (15.85±1.0) proliferation. Shoots were separated carefully and sub-cultured to induce roots
on ½ strength MS medium supplemented with IBA (1.5mg/l). Regenerated plants were successfully
hardened and acclimatized with 80 % survival rate under natural conditions after transplantation.

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Published

30.09.2010

How to Cite

UZMA RANI, NAJMA ISMAIL, GARIMA ZIBBU, & AMLA BATRA. (2010). HIGH FREQUENCY SHOOTS REGENERATION FROM IN VITRO RAISED SEEDLINGS OF MURRAYA KOENIGII (L.) SPRENG. International Journal of Pharma and Bio Sciences, 1(3), 1–7. Retrieved from https://ijpbs.net/index.php/journal/article/view/296

Issue

Volume 1, Issue 3, 2010

Section

Research Articles