A COMPUTATIONAL STUDY ON METALLOPROTEINASE IN THE SNAKE VENOMOUS OF DIFFERENT RATTLE SNAKE SPECIES IN COMPARISON WITH THE ASIAN SNAKE SPECIES

Abstract

Snake venom is a rich source of metalloproteinase which catalytic mechanism involves a metal after
snake bite is an important factor for created disease such as necrosis, heart disease, liver disease.
However, this is a very much important protein to design drug for treatment of various diseases. In this
study first we compared the amount of essential amino acids Histidine (H), Isoleucine (I), Leucine (L),
Lysine (K), Methioninne (M), Phenylalanine (F), Threonine (T), Tryptophan (W) and Valine (V) in
metalloproteinase four rattle snake where it was more (47.6%) in Crotalus adamanteus and less
(43.3%) in Sistrurus catenatus edwardsi. The multiple sequence alignment showed the highest
similarity between C. v. viridis and C. d. durissus (97%) and the lowest between C. adamanteus and S.
c. edwardsi (69%). Secondly we compared the percentage of essential amino acids in
metalloproteinase of rattle snake C. adamanteus with the Asian snake Naja atra (40.95%) and their
similarities was 55%. So rattle snake C. adamanteus contains more essential amino acids in
metalloproteinase. This result indicates that metalloproteinase could be useful to develop target
drug after isolation from venomous rattle snake species C. adamanteus.