ISOLATION, PRODUCTION AND CHARACTERIZATION OF EXTRACELLULAR PECTINASE FROM Aspergillus niger K3

Authors

  • ROHIT K C Sapthagiri College of Engineering, #14/5, Hesaraghatta Main Road, Bangalore – 560057, India
  • SUDIPTA K M Padmashree Institute of Management and Sciences, Kommagatta, Bangalore- 560060, India
  • PURUSHOTHAM B East West College of Science, Vishwaneedam Post, Bangalore- 560091, India
  • KUMARA SWAMY M Padmashree Institute of Management and Sciences, Kommagatta, Bangalore- 560060, India

Keywords:

Pectinase, pectin esterase, pectin, Aspergillus niger, ITS region.

Abstract

A pectinase producing fungus was isolated from compost soil. Morphological characteristics, microscopic observations and molecular characterization based on internal transcribed spacer (ITS) sequences led to its identification as Aspergillus niger (GenBank Accession Number: JX556221) and it was designated as Aspergillus niger strain K3. In the present study, the culture condition of the media was found to have a marked effect on pectinase production. The optimum pH and temperature for maximum pectinase activity was 8.0 (70U/ml) and 450C (90U/ml), respectively. The best carbon sources that induce pectinase production was found to be fructose (40U/ml) followed by maltose (38U/ml). Among the nitrogen sources, ammonium nitrate (80U/ml) produced maximum pectinase followed by peptone (72U/ml).

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Published

31.12.2013

How to Cite

ROHIT K C, SUDIPTA K M, PURUSHOTHAM B, & KUMARA SWAMY M. (2013). ISOLATION, PRODUCTION AND CHARACTERIZATION OF EXTRACELLULAR PECTINASE FROM Aspergillus niger K3. International Journal of Pharma and Bio Sciences, 4(4), 667–675. Retrieved from https://ijpbs.net/index.php/journal/article/view/2918

Issue

Volume 4, Issue 4, 2013

Section

Research Articles

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