Effect of the different auxins and cytokinins in callus induction, shoot, root regeneration in sugarcane

Authors

  • K.GOPITHA PG & Research Department of Biotechnology,Sengunthar Arts & Science College, Tiruchengode-637205, Namakkal District, Tamilnadu, India.
  • A.LAKSHMI BHAVANI PG & Research Department of Biotechnology,Sengunthar Arts & Science College, Tiruchengode-637205, Namakkal District, Tamilnadu, India.
  • J.SENTHILMANICKAM PG & Research Department of Biotechnology,Sengunthar Arts & Science College, Tiruchengode-637205, Namakkal District, Tamilnadu, India.

Keywords:

MS medium –Murashige and skoog medium BAP - 6-Benzyl amino purine; Kn - kinetin: IBA- Indole-3-Butyric acid: NAA -Napthalene acetic acid: CM - coconut milk

Abstract

Studies on micropropogation of callus culture was undertaken. In which expalnts were inoculated in MS medium fortified with various concentration of 2,4-D, auxin, cytokinin, sucrose at different pH level .The best callus induction was observed at 3.0mg/L, 2,4-D with 10% coconut milk (CM). Best regeneration of shoot was achieved when they were cultured on MS medium supplemented with BAP 1.0mg/L and IBA 0.5mg/L. Among the different media tested with 3mg/L NAA and 5% sucrose supplemented media proved best production of roots. 

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Published

30.09.2010

How to Cite

K.GOPITHA, A.LAKSHMI BHAVANI, & J.SENTHILMANICKAM. (2010). Effect of the different auxins and cytokinins in callus induction, shoot, root regeneration in sugarcane. International Journal of Pharma and Bio Sciences, 1(3), 1–7. Retrieved from https://ijpbs.net/index.php/journal/article/view/284

Issue

Volume 1, Issue 3, 2010

Section

Research Articles