EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ACONITUM HETEROPHYLLUM ROOT IN PARACETAMOL INDUCED LIVER TOXICITY

Abstract

The goal of the study was to evaluate the hepatoprotective activity of ethanolic extract of Aconitum heterophyllum root in paracetamol induced hepatic damage in Wistar albino rats. Thirty six albino rats weighing 150-200gr were selected and divided into six groups with 6 in each group.   Paracetamol (3gr/kg) was given to induce hepatotoxicity in the animals group II, III IV & V.  N-acetyl cysteine (100mg/kg) was given as standard drug in group III.  Group I given with normal saline. Group II given with paracetamol (3gm/kg) to induce hepatotoxicity. Group III was given paracetamol and standard drug N-Acetylcysteine (100mg/kg). Group IV and group V were given paracetamol and Aconitum heterophyllum (225mg/kg) and 450mg/kg respectively. Group VI was given plant extract root alone 450mg/kg. The hepatoprotective activity of ethanolic extract of Aconitum heterophyllum root was evaluated by the assessment of biochemical parameters such as serum glutamic oxaloacetic transaminases (SGOT), serum glutamic pyruvic transaminases (SGPT), alkaline phosphatise (ALP), total bilirubin, serum protein, and histopathological studies of the liver. Ethanolic extract of Aconitum heterophyllum root significantly reduced the liver damage and all biochemical parameters. The 450mg/kg extract showed greater response than 225mg/kg. This confirms the activity of ethanolic extract of Aconitum heterophyllum root has hepatoprotective activity.