IN VITRO SCREENING AND IDENTIFICATION OF ANTIOXIDANT ACTIVITIES OF ORANGE (CITRUS SINENSIS) PEEL EXTRACT IN DIFFERENT SOLVENTS

Abstract

Citrus plants are the rich source of secondary metabolites which includes alkaloids, steroids, tanins, flavonoids, phenols and polyphenols. Having the rich source of polyphenols and natural flavonoids draw attention to  screen the phytochemical compounds and their beneficial activities from different solvents. The present investigation explore the in vitro antioxidant activity of  orange (Citrus sinensis) peel extract from different solvents gives reliable information and best extraction procedures to  screen the phytochemicals. Among the different solvent extracts methonolic extract exhibits the highest  in vitro scavenging activity followed by other solvent extracts which includes Ethanolic, Hexane, Benzene, Ethyl acetate, and chloroform with IC₅₀ values 65.44,120, 138.45, 151.34, 170.34, 185.35 with 55.6 % inhibitory concentration of ascorbic acid. The screening and scavenging    activities of secondary metabolites form different solvents may improve the best extraction procedures and functional activities of beneficial compounds. In this regard to reach a conclusion that methonolic orange peel extract followed by ethonolic extract showed their high potential effectiveness as antioxidant activity when compared to the other solvent extracts.