Simultaneous Estimation of Paracetamol, Chlorzoxazone and Diclofenac Potassium in Pharmaceutical Formulation By A RP HPLC Method

Abstract

A fast and reliable high performance liquid chromatography method for determination of paracetamol, chlorzaxozone and diclofenac potassium has been developed. The chromatographic method was standardised using a reverse phase C18 column with UV-VIS detector at 254 nm. Mobile phase consisted of methanol 0.01M monobasic sodium phosphate with equal volume of 0.01M ortho phosphoric acid (70:30)  (pH adjusted to 2.5 ± 0.2 using 10% orthophosphoric acid) at a flow rate 1 ml/min. The method was validated and produced accurate and precise result for these drugs