BACTERIOLOGICAL PROFILE OF SURGICAL SITE INFECTION IN RURAL HOSPITAL IN R.R DISTRICT.

Abstract

Surgical site infections (SSI)are second most important nosocomial infections, As the prevalence is more in developing countries this study was conducted to know the bacteriological profile of surgical site infection, various predisposing factors, antibiogram. 200 swabs were collected from clinically diagnosed cases of surgical site infection. Two sterile cotton swabs were used to collect the samples, One for grams stain and other for culture. Culture done on Blood agar, MacConkeys agar, nutrient agar for isolation. Biochemical tests were done for further speciation. Antibiotic sensitivity done by Kirby-bauers disc diffusion test using Mueller Hinton agar according to standard guidelines. Detection MRSA  was done according to CLSI guidelines. Out of 200 samples,27 showed aerobic bacterial growth. Majority of SSI was by single organism either gram positive cocci or gram negative bacilli. Most common isolate was Staphylococcus aureus 11(40.7%) others were Klebsiella 07(25.9%),Pseudomonas 03(11.1%), E.coli 02(7.4%), Proteus, CoNS, Acinetobacter, Enterococci were 01(3.7%) each. SSI increasing because various factors like poor hygine ,emergence of drug resistant organism. Most common organism isolated from SSI was Staphylococcus aureus. MRSA noted 9.09% cases.SSI can be prevented by giving adequate antibiotic prophylaxis prior to operations. Proper hand wash in between dressing patients can cause significant reduction in number of cases SSI.