EFFECT OF DIFFERENT CYTOKININS ON SHOOT INDUCTION IN PIGEONPEA (CAJANUS CAJAN L.) MANAK (H77216) VARIETY

Authors

  • JHARNA SRIVASTAVA Department of Biotechnology and Food Sciences, Jayoti Vidyapeeth Women’s University, Jaipur, Rajasthan (India) – 303007
  • PRAMOD K. RAGHAV Department of Biotechnology and Food Sciences, Jayoti Vidyapeeth Women’s University, Jaipur, Rajasthan (India) – 303007
  • ANIRUDHA RISHI Department of Biotechnology and Food Sciences, Jayoti Vidyapeeth Women’s University, Jaipur, Rajasthan (India) – 303007

Keywords:

Cajanus cajan, cotyledonary node explant, MS media, BAP, Kinetin, Zeatin.

Abstract

Pigeonpea (Cajanus cajan [L] Millsp.) is an important grain legume of the semi-arid tropics. It provides protein rich food. Seeds of pigeonpea were collected and surface sterilized and soaked overnight. This sterilized seeds were decoated and inoculated in MS media with various combination of cytokinin for cotyledonary node explants. Seeds inoculated were used for morphogenic response and various frequencies were observed using (0mg/l,1mg/l,2mg/l,3mg/l and 4mg/l) concentration  of BAP, KIN and ZEA simultaneously. Highest percentage (88.35) of cotyledonary node explant formation was observed on MS media + 2.0 mg/l BAP, 3% sucrose, 0.7% (w/v) agar.

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Published

30.09.2013

How to Cite

JHARNA SRIVASTAVA, PRAMOD K. RAGHAV, & ANIRUDHA RISHI. (2013). EFFECT OF DIFFERENT CYTOKININS ON SHOOT INDUCTION IN PIGEONPEA (CAJANUS CAJAN L.) MANAK (H77216) VARIETY. International Journal of Pharma and Bio Sciences, 4(3), 55–63. Retrieved from https://ijpbs.net/index.php/journal/article/view/2549

Issue

Volume 4, Issue 3, 2013

Section

Research Articles

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