A NOVEL PROTOCOL DESIGN TO EVALUATE THE PREVENTIVE/ PROPHYLACTIC PROPERTY OF DRUGS/ PLANT PRODUCTS/ NEUTRACEUTICALS AGAINST EXPERIMENTALLY INDUCED IABETES IN ANIMAL MODELS

Abstract

To establish a novel methodology for the evaluation of prophylactic property of drugs/ plant products against experimentally induced diabetes in animal models. Test drug is administered by oral/ i.v. route to animals every day for 90 days. On 91st day, diabetes is induced by chemical method.  Test drug administration is continued for another 4 days i.e. from 91^st to 94^th day. CBG, lipid profile, body weight are measured once in 15 days till 90 days and everyday for next 10 days by any standard method. After 3^rd day of diabetes induction, test rats are not given any drugs, but regular feed is continued upto 10^th day. CBG is recorded on 2^nd/3^rd day after diabetes induction  and analysed as:If  average CBG/ lipid levels in the test group is :- i)Same or more compared to the diabetic control group: infers that there is no prophylactic  activity of the  evaluating  product. ii) Less than the diabetic control group: infers that the   test evaluating product has antihyperglycemic activity indicating the possibility of prophylactic property. ii) Less than the non-diabetic control group at any time of the experiment (1st to 94^th day):  infers that the evaluating product has a positive tendency of hypoglycemia with possibility of treatment property. This novel methodology of investigating a drug for its prophylactic property against chemically induced diabetes in animal models may be useful to evaluate a product with future perspective targeting preventive approach in prediabetics or high risk individuals of type 2 diabetes and normal individuals. This novel approach may be useful to screen   drugs / plant products / neutraceuticals likely to postpone onset of diabetes or development of its complications.