THE EFFICACY OF SPATHODEA CAMPANULATA P. BEAUV LEAF EXTRACT ON SOME BACTERIAL AND FUNGAL STRAINS BY WELL DIFFUSION METHOD

Abstract

The aim of the present study was to evaluate the qualitative analysis of phytochemicals and antimicrobial activity of chloroform, acetone, methanol and aqueous extracts of Spathodea campanulata. The antimicrobial activity of different extracts of S. campanulata was tested against the Gram-positive and Gram-negative bacterial strains and fungi by agar well diffusion method. The Gram- positive bacteria used in the test were S.aureus, B.subtilis, B.megaterium, S.mutans and the Gram-negative bacteria were E.coli, K.pneumoniae, P.aeruginosa, fungi like A.niger, A.flavus, T.viridae and C.albicans. It was observed that methanol extract exhibited maximum activity against S. mutans, P.aeruginosa, A. flavus and C.albicans. Chloroform and acetone extracts were exhibited moderate activity and aqueous extract exhibited less activity against the tested microorganisms. In this study different extracts of leaves of Spathodea campanulata exhibited varying degree of inhibition to the growth of tested organisms. The results confirmed the presence of antibacterial and antifungal activity of Spathodea campanulata leaf extracts against various human pathogenic microorganisms.