SCREENING AND IDENTIFICATIONIN VITRO ANTIOXIDANT ACTIVITIES OF PHYTOCHEMICAL COMPOUNDS IN ETHANOLIC GRAPE (VITIS VINIFERA) SEED EXTRACT

Abstract

Grape (Vitis vinifera) seed extract found to be exhibits wide range of beneficial effects insights to screen the potential compounds which includes polyphenolic compounds both flavonoids and non-flavonoids that show antioxidant effects. In this present study phytochemical screening of alkaloids, flavonoids, glycosides, polyphenol and flavonoids, tannins, and sterols were presented in the ethanolic grape seed extract. The In vitroantioxidant activity was estimated by IC₅₀ value and the values are65.44 µg/ml for DPPH radical scavenging assay and inhibition of hydroxyl radical, superoxide anions, hydrogen peroxide anions and lipid peroxide inhibition assay with IC₅₀ values of 77.33, 92.43, 88.15 and 83.54µg/ml, respectively. The results obtained from this study clearly indicate that ethanolic grape (Vitis vinifera) seed extract possesses antioxidant activity.