DEVELOPMENT AND VALIDATION OF NEW ANALYTICAL METHOD FOR PACLITAXEL IN BULK AND PHARMACEUTICAL DOSAGE FORM BY REVERSE PHASE HPLC (RP-HPLC)

Authors

  • SREERAMAMURTHY PYLA Raghu College of Pharmacy, Dakamarri (V), Bheemili (M), Visakhapatnam Dist. A.P, India.
  • K.SRINIVAS Sri Vasavi institute of pharmaceutical sciences, T.P.Gudem, E.G District.
  • JOGENDRAKUMAR YVV Raghu College of Pharmacy, Dakamarri (V), Bheemili (M), Visakhapatnam Dist. A.P, India.
  • JAGADEESH PANDA Raghu College of Pharmacy, Dakamarri (V), Bheemili (M), Visakhapatnam Dist. A.P, India.

Keywords:

Paclitaxel, RP- HPLC, Development, Validation.

Abstract

A simple, precise, rapid and accurate reverse phase HPLC method has been developed for the determination of Paclitaxel in bulk and its pharmaceutical dosage form. An enable C18G, 250mmX4.6mm i.d, 5µm particle size column was used with photo diode array UV-Visible detector. The mobile phase consisting of Phosphate Buffer pH 5.0 and Acetonitrile in the ratio of 90:10V/V was used. The flow rate was 1ml/min and the effluent was monitored at 282nm. The retention time of the drug was 3.610 minutes.  The method was linear over the concentration range of 25-125µg/ml. the method precision for the determination of assay was below 2% RSD. The percentage recovery of paclitaxel was 99.41 – 99.83%. The validation of method was carried out utilizing ICH guidelines.   

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Published

30.09.2013

How to Cite

SREERAMAMURTHY PYLA, K.SRINIVAS, JOGENDRAKUMAR YVV, & JAGADEESH PANDA. (2013). DEVELOPMENT AND VALIDATION OF NEW ANALYTICAL METHOD FOR PACLITAXEL IN BULK AND PHARMACEUTICAL DOSAGE FORM BY REVERSE PHASE HPLC (RP-HPLC). International Journal of Pharma and Bio Sciences, 4(3), 534–540. Retrieved from https://ijpbs.net/index.php/journal/article/view/2488

Issue

Volume 4, Issue 3, 2013

Section

Research Articles

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