REVERSE PHASE HPLC METHOD FOR QUANTITATIVE DETERMINATION OF EMBELIN IN POLY HERBAL FORMULATION

Authors

  • S.MAHESHWARAN Orchid Chemicals and Pharmaceuticals, Research and Development Centre, Sozhlinganallur, Chennai- 600 119.
  • C.SURESHKUMAR Asthagiri Herbal Research Foundation, 162A Perungudi industrial estate, Perungudi, Chennai-96.
  • R. MOHANKUMAR Inter disciplinary School of Indian System of Medicine, SRM University, Kanchipuram Dt. Kattankulathur - 603 203.
  • S. NARASIMHAN Asthagiri Herbal Research Foundation, 162A Perungudi industrial estate, Perungudi, Chennai-96.

Keywords:

Embelin; Quantitative analysis; Formulation; RP-HPLC; Validation.

Abstract

Embelin is a unique chemical compound found in nature, composed of quinone moiety resembling Coq10 (Ubiquinones), having ketone and hydroxy groups.  A Optimum solvent for extracting embelin from Embelia ribes and its simultaneous determination of content of embelin in the extract and content of embelin in Formulation which is available in market has been done in Gradient HPLC with less runtime of 10 minutes.  Satisfactorily the results were obtained with respect to LOQ (0.5ppm), LOD (0.17 ppm) and Linearity (Correlation: 0.9976), Ruggedness (RSD: 0.8%), Accuracy (86%), Precision (RSD: 1.0%).  The Present method was sensitive, accurate, simple reproducible and therefore can be recommended for using in Quality control to check the content of Embelin.

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Published

30.09.2013

How to Cite

S.MAHESHWARAN, C.SURESHKUMAR, R. MOHANKUMAR, & S. NARASIMHAN. (2013). REVERSE PHASE HPLC METHOD FOR QUANTITATIVE DETERMINATION OF EMBELIN IN POLY HERBAL FORMULATION. International Journal of Pharma and Bio Sciences, 4(3), 116–123. Retrieved from https://ijpbs.net/index.php/journal/article/view/2439

Issue

Volume 4, Issue 3, 2013

Section

Research Articles

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