A NEW TITRIMETRIC METHOD FOR THE ASSAY OF SOME ANTINEOPLASTIC AGENTS

Abstract

In the present work we have developed a new method for the determination of some
Antineoplastic agents i.e. capecitabine, cytarabine, etoposide, doxorubicin
hydrochloride and methotrexate in pure form and in their pharmaceutical preparations
like xabine (Tab.), xeloda (Tab.), cytabine (Inj.), cytaraside (Vial), etosid (Tab.), peltasol
(Inj.), zodox (Vial), oncodox (Inj.), biotrexate (Inj.), and imutrex (Inj.) by using Nchlorosuccinimide
(NCS) reagent in acidic medium. This reagent in acidic medium
produces chloronium ion (Cl+) which is highly reactive. It may react with allylic hydrogen
or hydroxyl group of the substances and forming respective oxidation products. Aliquots
containing 1-5 mg of sample were taken in 100 mL erlenmeyer flask followed by the
addition of 5mL of 0.02N N-chlorosuccinimide reagent (NCS) and 5mL of 4N
hydrochloric acid. The reaction mixture was shaken thoroughly and allowed to react at
room temperature (25-300C) for required reaction time (10min.). After the reaction was
over 5mL of 5% KI solution was added to it, contents shaken thoroughly and allowed to
stand for a minute. The liberated iodine was titrated against 0.02N sodiumthiosulphate
solution using starch indicator. A blank experiment was also run under identical
conditions using all the reagents except sample. Results were calculated by the
difference in the titre value of 0.02N sodiumthiosulphate for blank and actual
experiment. Maximum deviation was found within the error of ± 1%. The value of
standard deviation (SD), relative standard deviation (RSD) and recovery experiments
prove to be precise and reproducible.