ANTIBACTERIAL INVESTIGATED OF PHOSPHOLIPASE A2FROM THE SPINES VENOM OF CROWN OF THORNS STARFISH ACANTHASTER PLANCI

Authors

  • FERA IBRAHIM Research and Healthcare Service Center on Virology and Cancer Pathobiology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia.
  • NUNUK WIDHYASTUTI Research Center for Biotechnology, Indonesian Institute of Science, Cibinong 1691, Indonesia.
  • IMELDA KRISANTA ENDA SAVITRI Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia Department of Marine Product Technology, Faculty of Fishery and Marine Science, Pattimura University, Ambon 97233, Indonesia
  • MUHAMAD SAHLAN Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia
  • ANONDHO WIJANARKO Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia

Keywords:

Phospholipase A2, venom, Acanthaster planci, antibacterial activity

Abstract

Phospholipase A2 isolated from venoms is a potential new antibacterial source.   Antibacterial activity tests of phospholipase A2 were performed against Staphylococcus aureus and Bacillus subtilis. Phospholipase A2 which was isolated from spines venom of Acanthaster planci by using fractionated 20% saturated ammonium sulfate precipitation and its antibacterial assay was done by using disc diffusion method whereas 40 μl isolate at a concentration of 6.29 μg/μl was applied onto 7 mm paper disc. The assay exhibited inhibition zone around the phospholipase A2disc against Staphylococcus aureus while no inhibition zone against Bacillus subtilis. As a result, phospholipase A2 posses an antibacterial activity against Staphylococcus aureus.

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Published

30.06.2013

How to Cite

FERA IBRAHIM, NUNUK WIDHYASTUTI, IMELDA KRISANTA ENDA SAVITRI, MUHAMAD SAHLAN, & ANONDHO WIJANARKO. (2013). ANTIBACTERIAL INVESTIGATED OF PHOSPHOLIPASE A2FROM THE SPINES VENOM OF CROWN OF THORNS STARFISH ACANTHASTER PLANCI. International Journal of Pharma and Bio Sciences, 4(2), 1–5. Retrieved from https://ijpbs.net/index.php/journal/article/view/2263

Issue

Volume 4, Issue 2, 2013

Section

Research Articles

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