INVITRO EVALUATION OF α-AMYLASE INHIBITORY ACTIVITY & ANTIOXIDANT POTENTIAL OF PTERIS VITTATA L. WITH SPECIAL REFERENCE TO ITS HPTLC PROFILE.

Abstract

Pteris vittata L., a fern was evaluated for its alpha amylase inhibitory potential owing to its previously reported antihyperglycemic activity. This Pteridophyte was also assayed for its free radical inhibition ability in ABTS decolourisation assay followed by quantification and HPTLC of polyphenols. The Porcine pancreatic alpha amylase inhibition was studied invitro and compared with standard drug Acarbose and their IC50 values were determined. For Antioxidant Potential, the ABTS free Radical Scavenging activity was performed and concentration of the test extracts equivalent to ascorbic acid was determined. The phenols and flavonoids being held responsible for antioxidant propertiesof  plants lead us to quantify them which were followed by HPTLC fingerprinting of flavonoids to facilitate its identification. Standard Quercetin was used as standard along with the two extracts of Pteris vittata L. As compared to aqueous extract, the ethanolic extract showed better results in the parameters undertaken to study.