THE PROTECTIVE EFFECT OF CO-SUPPLEMENTATION OF N-ACETYL CYSTEINE AND MAGNESIUM AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN ALBINO RAT

Abstract

Increased production of reactive oxygen species (ROS) may be a common pathway in atherosclerosis, diabetes, cataract, accelerated ageing, and drug toxicities. The protective role of N-acetyl cysteine (NAC) and magnesium (Mg⁺²) in gentamicin induced nephrotoxicity was evaluated . One hundred albino rats were divided into 5 groups. Sham control group (A), Gentamicin group (B), Gentamicin + NAC group (C), Gentamicin + Mg⁺²  group ( D), and  gentamicin + (NAC + Mg⁺²) group  (E) . Twenty four  hour urinary albumin excretion (UAE), plasma  creatinine (crP),  superoxide dismutase (SOD), catalase (CAT) ,reduced glutathione(GSH) , and malondialdehyde (MDAP) were measured. CrP ,UAE and MDAP were significantly increased in gentamicin treated group, while  SOD, CAT , and  GSH were significantly decreased compared with those in the control group.  Combination between NAC and Mg⁺² produced  a significant decrease of  the lipid peroxidation production and increase of the antioxidant enzymes. Increased lipid peroxidation and decreased antioxidant enzymes may play a role in gentamicin induced renal toxicity. Combined NAC + Mg⁺²  may ameliorate these changes and protect the kidney.