HPTLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF 6-GINGEROL IN ORAL THIN FILM

Abstract

A HPTLC method was established for assay of 6-Gingerol and determination from herbal oral thin film (OTF).The chromatographic condition were: Toluene: Ethyl acetate: methanol: Formic Acid (5: 4:1:1v/v/v/v) as mobile phase; wavelength of the detector was 520nm; drying temperature after development was 60°C. The calibration curve was linear in the range of 100 to 600mcg/ml. With correlation coefficient of 0.999; the average recovery was found to be 100.24%, 100.45% and 100.49% at the concentration levels of 80, 100 and 120% respectively. Detection and quantification limit was fond to 80 and 120ng. The method was found to be simple, reliable, accurate and precise in accordance with ICH guidelines.