EFFECT OF PROBIOTIC STRAIN LACTOBACILLUS CASEISTRAIN 17 AGAINST TOXICITYINDUCED BY CHROMIUM IN FEMALE REPRODUCTIVE SYSTEM OF RATS.

Abstract

The present study was aimed to investigate whether Lactobacillus casei strain17 could protect the chromium (Cr) VI-induced oxidative stress in female reproductive system of rats and to explore the underlying mechanism of the same. A total of 24 Wistar adult female rats were equally divided into four groups. Group 1 served as control, while groups 2 and 3 were administered K₂Cr₂O₇ (10 mg/kg b.wt. s.c. single dose). In addition to Cr, group 3 also received Lactobacillus casei(Lc) strain 17 at the rate of 1 x 10⁹ CFU/rat/day for 14 days. Group 4 was maintained as probiotic control (dose as above). Body weights were recorded at the beginning and at the end of experiment. Further the rats were observed for occurrence of estrus cycle.  At the end of 14 days, blood samples were drawn for sero-biochemical analysis. Subsequently, all the rats were sacrificed to collect uterus along with ovaries for assay of tissue peroxidation, antioxidant, functional markers and histopathology. Administration of chromium (Cr) VI to rats revealed a significant (p<0.05) accumulation of cholesterol and a prolonged diestrus phase leading to fertility impairment in rats.Administration of chromium (Cr) VI significantly (p<0.05) reduced the antioxidant markers such as superoxide dismutase (SOD) and reduced glutathione (GSH), along with significant (p<0.05) increase in peroxidation markers such as malondialdehyde and protein carbonyls in ovaries. The functional markers in serum such as total protein were decreased whereas other functional markers vizalanine transaminase (ALT), blood urea nitrogen (BUN) and creatinine were increased.  Prominent pathological changes were observed in the uterus and ovaries of Cr-treated group. Co-treatment with Lactobacillus casei(Lc) strain 17 significantly (p<0.05) reversed the (Cr) VI induced changes.