GLYCINE PROPIONYL L-CARNITINE (GPLC) AMELIORATES D-GALACTOSAMINE (D-GALN) INDUCED OXIDATIVE STRESS IN RATS BY PREVENTING LIPID PEROXIDATION AND MAINTAINING THE REDOX POTENTIAL.

Abstract

Evaluation of the role of Glycine Propionyl L-Carnitine (GPLC) in ameliorating D-Galactosamine (D-GalN) induced oxidative stress in male wistar rats. Methods: Our current study included the treatment of male wistar rats with GPLC and D-GalN. Animals were given a pretreatment of GPLC for two months at a dose of 35mg/kgBW/day after which a single dose of D-GalN (700 mg/kgBW) was administered (ip). Another group was administered with D-GalN alone. Hepatic biochemical markers of oxidative stress (ALP,AST,ALT, γ- GT ,GSH,MDA,SOD,CAT and TNF-α) were monitored after the administration of D-GalN at 0 hr, 24 hr and 48 hr in all the groups,. Results: ALP, AST, ALT and γ- GT levels in control group were measured to be 70.51 ± 1.31, 66.83 ± 1.93, 72.65 ± 1.92 and 8.90 ± 0.83 and when compared with D-GalN treated (alone) group gave P < 0.001. Similarly GSH, MDA, SOD, CAT and TNF-α pool in control group were measured to be 46.80 ± 0.98, 8.14 ± 0.54, 14.86 ± 0.85, 80.81 ± 1.30, 26.90 ± 0.86 and when compared with D-GalN treated group gave P < 0.001.On the other hand GPLC, when administered simultaneously with D-GalN, showed a significant protection against D-GalN induced damage as the values of biochemical markers when compared to the D-GalN group gave P < 0.001.  Conclusion: GPLC is able to prevent the lipid peroxidation by ROS. Dietary supplement of GPLC could prove to be a better neutraceutical having antioxidant role against hepatotoxicants. Moreover it maintains the redox potential inside a cell due to its radical scavenging action thereby maintaining a healthy state of an individual.