PHB PRODUCTION BY BACILLUS SPECIES USING THE CHEAP SUBSTRATE GROUNDNUT OIL CAKE

Abstract

The present study encomparies of the isolation and production of PHB (Poly-β-hydroxy-butyrate) producing Bacillus sp., from soil samples. The accumulation of PHB as granules in the organism was analyzed by Sudan black staining method. The PHB production was determined from the selected strains (B6, B7, B8, B10 and B11) by crotonic acid extraction method using U-V spectrophotometer at 235nm. The optimization of pH and temperature were determined by standard methods. Maximum amount of PHB were obtained by standardizing the physical parameters such as temperature at 37ËšC and pH at 7. The cheap substrate Groundnut oil cake was used as a carbon source. The substrate Groundnut oil cake optimization at different concentrations (1%, 2%, 3%, 4% and 5%). Out of all these concentrations, the strains under 5% were considered as highest production of PHB. On comparing with the PHB production at 4%, showed the slight increased production rate was observed at 5%. The sugar content of Groundnut oil cake was standardized with standard DNSA method. IR (FTIR) spectra revealed the presence of PHB functional groups, showed 3 major and 3 minor peaks were observed at 235nm.