DETECTION OF PUNGENT AND NON-PUNGENT CHILLI PEPPER (CAPSICUM SP.) VARIETIES AT SEED LEVEL, USING PUN ALLELE SPECIFIC PRIMER MAP1

Authors

  • BYLLA PRASAD Department of Biotechnology, Kakatiya University, Warangal-506 009, India
  • TOUTREDDY RADHA Department of Biotechnology, Kakatiya University, Warangal-506 009, India
  • ROHELA GULAB KHAN Department of Biotechnology, Kakatiya University, Warangal-506 009, India
  • CHITHAKARI RAVI Department of Botany, Kakatiya University, Warangal-506 009, India
  • THAMMIDALA CHRISTOPHER REUBEN Department of Botany, Kakatiya University, Warangal-506 009, India

Keywords:

Capsicum annuum L., Capsicum chinense, Jacq, MAP1 primer, zygotic embryo, genomic DNA, PCR.

Abstract

Genomic DNA isolated from zygotic embryos (seeds) of 7 chilli pepper varieties, namely Capsicum annuum L., California wonder, Yolo wonder, G-5, Pusa sadabahar, Pusa jyothi and LCA-335 and Capsicum chinense Jacq. variety Kotpar, was PCR-amplified using Pun1 allele specific primer MAP1.  A DNA band admeasuring 479 bp was observed in C annuum L., varieties California wonder and Yolo wonder, while a DNA band admeasuring 494 bp was observed in the remaining 5 varieties. This polymorphism was used to discriminate non-pungent varieties namely California wonder and Yolo wonder from pungent varieties namely G-5, Pusa sadabahar, Pusa jyothi, LCA-335 and Kotpar. This method is useful to distinguish non-pungent varieties from pungent varieties, at seed level itself.

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Published

31.03.2013

How to Cite

BYLLA PRASAD, TOUTREDDY RADHA, ROHELA GULAB KHAN, CHITHAKARI RAVI, & THAMMIDALA CHRISTOPHER REUBEN. (2013). DETECTION OF PUNGENT AND NON-PUNGENT CHILLI PEPPER (CAPSICUM SP.) VARIETIES AT SEED LEVEL, USING PUN ALLELE SPECIFIC PRIMER MAP1. International Journal of Pharma and Bio Sciences, 4(1), 889–892. Retrieved from https://ijpbs.net/index.php/journal/article/view/2104

Issue

Volume 4, Issue 1, 2013

Section

Research Articles

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