IN-VITRO ANTIMICROBIAL ACTIVITY AND IN-VIVO TOXICITY OF MORINGA OLEIFERA AND ALLAMANDA CATHARTICA AGAINST MULTIPLE DRUG RESISTANT CLINICAL PATHOGENS

Abstract

Based on the traditional usage, Moringa oleifera and Allamanda cathartica plants were selected. The present study is to find out its antimicrobial potential against clinical isolates of KMCHospital. The resistant patterns of the isolated pathogens were studied against commercial standard antibiotics. By using the different polar solvents (Methanol, Ethanol, Chloroform, Water, Petroleum ether and Ethyl acetate), the plants material were extracted and studied their phytochemical constituents. Antimicrobial activity of plants extract were studied by well diffusion assay in muller hinton agar and minimum inhibitory concentration of the significant extracts were studied by tube dilution assay. The results of A. cathartica antimicrobial property revealed that petroleum ether extract showed good inhibition (Staphylococcus aureus (20mm), Escherichia coli (13mm), Pseudomonas aeruginosa (19mm), Acinetobacter sp (20mm), Proteus sp (18mm)) than that of remaining extracts. M. oleifera ethanol extract showed significant inhibition (S.aureus (20mm), E.coli (20mm), Bacillus sp (18mm), P. aeruginosa (14mm), Cornebacterium  sp (12mm), Klebsiella  pneumonia (12mm), Acinetobacter sp (14mm)). Acute toxicity was performed in albino mice and  up to 1000mg/kg, no toxic symptoms were observed. These results suggested that both the plants showed relatively broad spectrum of antimicrobial activity and are non toxic.