EFFECT OF SAPINDUS MUKOROSSI SAPONIN FRACTION AND ANTHRAQUINONE FRACTION OF RHEUM EMODI ON CCL4 INDUCED CYTOCHROME P450 DAMAGE IN WISTAR RATS

Abstract

The cytochrome P₄₅₀ protective activity of Sapindus mukorossi and Rheum emodi were evaluated against CCl₄ induced prolongation of pentobarbitone sleep time in Wister rats. The fractions, at a dose of 0.2 g/kg,0.4g/kg p.o., Sapindus mukorossi, Rheum emodi significantly prevents the CCl₄ induced prolongation of pentobarbitone sleep time (P<0.01), indicating the cytochrome P₄₅₀ protection activity. However, when treated alone, the fractions (0.2 g/kg and 0.4 g/kg, p.o.) shows a significant prolongation of pentobarbitone sleep time (P<0.01), indicating the intrinsic cytochrome P₄₅₀ inhibition activity. In addition, the extracts show neither stimulant nor depressant effect on the CNS, as evident from locomotor activity test. In conclusion, the results indicate that, the Sapindus mukorossi saponin fraction and anthraquinone fraction of rheum emodi at an oral dose of 0.2 g/kg and 0.4 g/kg, p.o., shows significant protective effects against CCl₄ induced cytochrome P₄₅₀ damage and also show a significant intrinsic cytochrome P₄₅₀ inhibition activity.