PHOTO-OXIDATION AND QUINACRINE FLUORESCENCE RESPONSE IN THE MITOTIC CHROMOSOMES OF THE FLESH-FLIES SARCOPHAGA

Authors

  • RASHMI SRIVASTAVA Department of Zoology, University of Allahabad, Allahabad- 211002, India Current address: National Bureau of Fish Genetic Resources, Lucknow-226001, India
  • PRATIMA GAUR Department of Zoology, University of Allahabad, Allahabad- 211002, India
  • NIDHI MISHRA Department of Zoology, University of Allahabad, Allahabad- 211002, India

Keywords:

Mitotic chromosomes, Sarcophaga species, Quinacrine, photo-oxidation

Abstract

Experiments have been carried out to investigate the DNA base specificity of light-induced banding (LIB) produced by photo-oxidation of chromosomes followed by Quinacrine staining to detect denatured DNA. In the present study mitotic preparations from Sarcophaga ruficornis and Sarcophaga argyrostoma were used to detect the visible light and fluorochrome interactions. Our results indicated that specific destruction of guanine residues is the main effect of photo-oxidation and LIB is a base-specific phenomenon. In addition, photo-oxidation may also cause DNA-protein cross-linking which affects the binding of some dyes, while prolonged photo-oxidation appears to cause more general damage to DNA.

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Published

31.03.2013

How to Cite

RASHMI SRIVASTAVA, PRATIMA GAUR, & NIDHI MISHRA. (2013). PHOTO-OXIDATION AND QUINACRINE FLUORESCENCE RESPONSE IN THE MITOTIC CHROMOSOMES OF THE FLESH-FLIES SARCOPHAGA. International Journal of Pharma and Bio Sciences, 4(1), 250–255. Retrieved from https://ijpbs.net/index.php/journal/article/view/2019

Issue

Volume 4, Issue 1, 2013

Section

Research Articles

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