PRODUCTION OF POLY (3- HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE) BY A NOVEL BACILLUS OU40T FROM INEXPENSIVE CARBON SOURCES

Abstract

The goals of this work are to characterize and evaluate the ability of strain OU40^T to produce poly (3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV) using various agricultural wastes, isolated from polluted water.Growth of the isolate OU40^T was assessed in mineral media containing glucose, starch, bagasse, whey and rice bran without adding any precursors.  Biopolymers produced by strain OU40^T had an average molecular weight 1,304 kDa to 2,332 kDa with the melting point 155-160 T_m(^oC) and percentage of crystallinity 40- 50%. Based on phenotypical, biochemical and genotypic investigations,  strain OU40^T is assigned to the genus Bacillus. The DNA-DNA relatedness between OU40^T and Bacillus cereus ATCC14579^T (AE016877) was found 60% of mean similarity, 4.24 SD value,  ΔTm value of  6% and  G+C content was 46.5%.These result suggests that the strain could be a novel genomic species.The 16S rRNA gene sequence is deposited in EMBL/Genbank with accession number FN663629.The type strain is OU40^T (=JCM17287^T=CCM7835^T =DSM24141^T).