PRECISE METHOD FOR THE ASSESSMENT OF CATALASE-LIKE ACTIVITY IN SEMINAL FLUIDS

Authors

  • MAHMOUD HUSSEIN HADWAN Chemistry Dept., College of Science, Babylon University, Iraq.
  • LAMIA A. ALMASHHEDY Chemistry Dept., College of Science, Babylon University, Iraq.
  • ABDUL RAZZAQ S. ALSALMAN Surgery Dept., College of Medicine, Babylon University, Iraq.

Keywords:

catalase-like activity; hydrogen peroxide; seminal plasma; ammonium molybdate.

Abstract

In the present study, a sensitive modification of the colorimetric assay for catalase activity is reported. This assay is based on the reaction of undecomposed hydrogen peroxide with ammonium molybdate to give a yellowish color, which has maximum absorbance at 354 nm. Catalase-like activity is calculated from a comparison with the activity of a certified standard enzyme with known unit activity (50 k.units/litre). The seminal plasma catalase activity of a sample of normal individual has been determined. The imprecision of the method was calculated by measuring the coefficient of variation, which equals to 2.61% within a run and 5.35% between run.  The catalase assay performed using kinetic method yielded a good correlation (r = 0.98504).

Downloads

Published

31.03.2013

How to Cite

MAHMOUD HUSSEIN HADWAN, LAMIA A. ALMASHHEDY, & ABDUL RAZZAQ S. ALSALMAN. (2013). PRECISE METHOD FOR THE ASSESSMENT OF CATALASE-LIKE ACTIVITY IN SEMINAL FLUIDS. International Journal of Pharma and Bio Sciences, 4(1), 949–954. Retrieved from https://ijpbs.net/index.php/journal/article/view/1982

Issue

Volume 4, Issue 1, 2013

Section

Research Articles

Categories