ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ASSAY OF AMIFOSTINE FOR INJECTION BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Authors

  • BASHEER AHMAD.S Nimra College of Pharmacy, Nimra Nagar, Ibrahimpatnam, Vijayawad-521456
  • HARITHA PAVANI.K Nimra College of Pharmacy, Nimra Nagar, Ibrahimpatnam, Vijayawad-521456
  • SURESH BABU.U Nimra College of Pharmacy, Nimra Nagar, Ibrahimpatnam, Vijayawad-521456
  • HAIMA.R Nimra College of Pharmacy, Nimra Nagar, Ibrahimpatnam, Vijayawad-521456
  • SHIVAJI.K Nimra College of Pharmacy, Nimra Nagar, Ibrahimpatnam, Vijayawad-521456

Keywords:

Amifostine for injection, RP-HPLC, Validation.

Abstract

A simple, economic, selective, precise, and accurateReverse phase High Performance Liquid Chromatography method for analysis of Amifostine for injection, was developed and validated according to ICH guidelines.  The quantification of the drug was carried out using Lunar C­­8, 250 mm X 4.6 mm, 5 mm columnor its equivalent in isocratic mode, with mobile phase compressing of Buffer: Methanol (72:28)the flow rate was 1.0ml/min and the detection was carried at 220 nm. The retention time for Amifostine for injection was found to be 4.5. The percent assay was found to be 97.3%. Proposed method was validated for precision, accuracy, linearity range, specificity and robustness. 

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Published

31.03.2013

How to Cite

BASHEER AHMAD.S, HARITHA PAVANI.K, SURESH BABU.U, HAIMA.R, & SHIVAJI.K. (2013). ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ASSAY OF AMIFOSTINE FOR INJECTION BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. International Journal of Pharma and Bio Sciences, 4(1), 722–727. Retrieved from https://ijpbs.net/index.php/journal/article/view/1957

Issue

Volume 4, Issue 1, 2013

Section

Research Articles

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